› 4x0n

X-ray diffraction
2.6Å resolution

Porcine pancreatic alpha-amylase in complex with helianthamide, a novel proteinaceous inhibitor

Released:
DOI: 10.2210/pdb4x0n/pdb
PDB entry 4x0n coloured by chain, front view.
PDB entry 4x0n coloured by chain, side view.
PDB entry 4x0n coloured by chain, top view.
Source organisms:
Entry authors: Williams LK, Brayer GD

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102796 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pancreatic alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 496 amino acids
Theoretical weight: 55.37 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00690 (Residues: 16-511; Coverage: 100%)
Gene name: AMY2
Sequence domains:
Structure domains:
Alpha-amylase inhibitor helianthamide Chain: B
Molecule details ›
Chain: B
Length: 44 amino acids
Theoretical weight: 4.7 KDa
Source organism: Stichodactyla helianthus
Expression system: Not provided
UniProt:
  • Canonical: A0A0X1KGZ5 (Residues: 1-44; Coverage: 100%)

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand CA 1 x CA
Ligand GOL 1 x GOL
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 43.75Å b: 103.28Å c: 111.896Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.226
Expression system: Not provided

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