› 4x0x

X-ray diffraction
1.9Å resolution

The structure of AhpE from Mycobacterium tuberculosis revisited

Released:
DOI: 10.2210/pdb4x0x/pdb
PDB entry 4x0x coloured by chain, front view.
PDB entry 4x0x coloured by chain, side view.
PDB entry 4x0x coloured by chain, top view.
Source organism: Mycobacterium tuberculosis CDC1551
Primary publication:
Revisiting sulfur H-bonds in proteins: The example of peroxiredoxin AhpE.
van Bergen LA, Alonso M, Palló A, Nilsson L, De Proft F, Messens J
OpenAccess logo Sci Rep 6 30369 (2016)
PMID: 27468924

Function and Biology Details

Reaction catalysed: 
Mycoredoxin + ROOH = mycoredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkyl hydroperoxide reductase E Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 154 amino acids
Theoretical weight: 16.97 KDa
Source organism: Mycobacterium tuberculosis CDC1551
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIE2 (Residues: 1-153; Coverage: 100%)
Gene names: MT2298, ahpE
Sequence domains: AhpC/TSA family
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P42
Unit cell:
a: 148.199Å b: 148.199Å c: 33.864Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.249
Expression system: Escherichia coli

Quick links

Citations

2 review citations

C-H⋯S hydrogen bonding interactions.
Fargher et al. (2022)
1 more

3 mentions without citation

Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities.
Bolduc et al. (2021)
2 more