› 4xc3

X-ray diffraction
1.63Å resolution

Crystal structure of human 4E10 Fab in complex with its peptide epitope on HIV-1 gp41; crystals cryoprotected with rac-glycerol 1-phosphate

Released:
DOI: 10.2210/pdb4xc3/pdb
PDB entry 4xc3 coloured by chain, front view.
PDB entry 4xc3 coloured by chain, side view.
PDB entry 4xc3 coloured by chain, top view.
Source organisms:
Primary publication:
Crystallographic Identification of Lipid as an Integral Component of the Epitope of HIV Broadly Neutralizing Antibody 4E10.
Irimia A, Sarkar A, Stanfield RL, Wilson IA
Immunity 44 21-31 (2016)
PMID: 26777395

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209262 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
4E10 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
4E10 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 230 amino acids
Theoretical weight: 24.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Transmembrane protein gp41 Chain: P
Molecule details ›
Chain: P
Length: 16 amino acids
Theoretical weight: 2.2 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: P05877 (Residues: 672-684; Coverage: 2%)
Gene name: env

Ligands and Environments

2 bound ligands:
Ligand 1GP 2 x 1GP
Ligand UNL 1 x UNL
1 modified residue:
Ligand 41H 1 x 41H

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 157.541Å b: 44.704Å c: 85.942Å
α: 90° β: 113.22° γ: 90°
R-values:
R R work R free
0.139 0.137 0.18
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

14 review citations

Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design.
Burton et al. (2016)
13 more

2 mentions without citation

Crystallographic Identification of Lipid as an Integral Component of the Epitope of HIV Broadly Neutralizing Antibody 4E10.
Irimia et al. (2016)
1 more