› 4xgx

X-ray diffraction
1.9Å resolution

Crystal structure of Escherichia coli Flavin trafficking protein, an FMN transferase, Y60N mutant, ADP-inhibited

Released:
DOI: 10.2210/pdb4xgx/pdb
PDB entry 4xgx coloured by chain, front view.
PDB entry 4xgx coloured by chain, side view.
PDB entry 4xgx coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Molecular insights into the enzymatic diversity of flavin-trafficking protein (Ftp; formerly ApbE) in flavoprotein biogenesis in the bacterial periplasm.
Deka RK, Brautigam CA, Liu WZ, Tomchick DR, Norgard MV
OpenAccess logo Microbiologyopen 5 21-38 (2016)
PMID: 26626129

Function and Biology Details

Reaction catalysed: 
FAD + [protein]-L-threonine = [protein]-FMN-L-threonine + AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD:protein FMN transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 37.6 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AB85 (Residues: 21-351; Coverage: 100%)
Gene names: JW5368, apbE, b2214, yojK, yojL
Sequence domains: ApbE family
Structure domains: ApbE-like domains

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand ADP 2 x ADP
Ligand NO3 9 x NO3
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 54.583Å b: 56.981Å c: 224.766Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Flavin transferase: the maturation factor of flavin-containing oxidoreductases.
Bogachev et al. (2018)
15 other articles

1 mention without citation

Molecular insights into the enzymatic diversity of flavin-trafficking protein (Ftp; formerly ApbE) in flavoprotein biogenesis in the bacterial periplasm.
Deka et al. (2016)