› 4xjn

X-ray diffraction
3.11Å resolution

Structure of the parainfluenza virus 5 nucleocapsid-RNA complex: an insight into paramyxovirus polymerase activity

Released:
DOI: 10.2210/pdb4xjn/pdb
PDB entry 4xjn coloured by chain, front view.
PDB entry 4xjn coloured by chain, side view.
PDB entry 4xjn coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the paramyxovirus parainfluenza virus 5 nucleoprotein-RNA complex.
Alayyoubi M, Leser GP, Kors CA, Lamb RA
Proc Natl Acad Sci U S A 112 E1792-9 (2015)
PMID: 25831513

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183415 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleoprotein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M
Length: 525 amino acids
Theoretical weight: 58.4 KDa
Source organism: Mammalian orthorubulavirus 5
Expression system: Escherichia coli
UniProt:
  • Canonical: Q88435 (Residues: 1-509; Coverage: 100%)
Gene names: N, NP
Sequence domains: Paramyxovirus nucleocapsid protein
RNA (78-MER) Chain: N
Molecule details ›
Chain: N
Length: 78 nucleotides
Theoretical weight: 23.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand PB 14 x PB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 205.636Å b: 309.436Å c: 233.241Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.262
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Polymerases of paramyxoviruses and pneumoviruses.
Fearns et al. (2017)
15 more

20 mentions without citation

Nucleocapsid Structure of Negative Strand RNA Virus.
Luo et al. (2020)
19 more