› 4xmf

X-ray diffraction
1.6Å resolution

Crystal structure of nitrophorin 7 from Rhodnius prolixus at pH 7.8 complexed with histamine

Released:
DOI: 10.2210/pdb4xmf/pdb
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Source organism: Rhodnius prolixus
Primary publication:
Structure and dynamics of the membrane attaching nitric oxide transporter nitrophorin 7.
Knipp M, Ogata H, Soavi G, Cerullo G, Allegri A, Abbruzzetti S, Bruno S, Viappiani C, Bidon-Chanal A, Luque FJ
OpenAccess logo F1000Res 4 45 (2015)
PMID: 26167269

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-7 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.76 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6PQK2 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand HSM 1 x HSM
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 38.338Å b: 66.722Å c: 38.825Å
α: 90° β: 116.87° γ: 90°
R-values:
R R work R free
0.161 0.158 0.207
Expression system: Escherichia coli

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Citations

6 citation in other articles

Mycobacterial and Human Ferrous Nitrobindins: Spectroscopic and Reactivity Properties.
De Simone et al. (2021)
5 more

1 mention without citation

Structure and dynamics of the membrane attaching nitric oxide transporter nitrophorin 7.
Knipp et al. (2015)

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