› 4xoe

X-ray diffraction
2.4Å resolution

Crystal structure of a FimH*DsG complex from E.coli F18 with bound heptyl alpha-D-mannopyrannoside

Released:
DOI: 10.2210/pdb4xoe/pdb
PDB entry 4xoe coloured by chain, front view.
PDB entry 4xoe coloured by chain, side view.
PDB entry 4xoe coloured by chain, top view.
Source organism: Escherichia coli 536
Primary publication:
Catch-bond mechanism of the bacterial adhesin FimH.
Sauer MM, Jakob RP, Eras J, Baday S, Eriş D, Navarra G, Bernèche S, Ernst B, Maier T, Glockshuber R
OpenAccess logo Nat Commun 7 10738 (2016)
PMID: 26948702

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-495134 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
FimH protein Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 29.05 KDa
Source organism: Escherichia coli 536
Expression system: Escherichia coli
Structure domains: Fimbrial-type adhesion domain
FimG protein Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.42 KDa
Source organism: Escherichia coli 536
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A140UH97 (Residues: 1-14; Coverage: 100%)
Gene name: ECP_4654

Ligands and Environments

2 bound ligands:
Ligand KGM 1 x KGM
Ligand CAC 1 x CAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P213
Unit cell:
a: 128.36Å b: 128.36Å c: 128.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.179
Expression system: Escherichia coli

Quick links

Citations

21 review citations

UroPathogenic Escherichia coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-antibiotic Antimicrobial Strategies.
Terlizzi et al. (2017)
20 more

13 mentions without citation

Glycomimetics for the inhibition and modulation of lectins.
Leusmann et al. (2023)
12 more