› 4y0v

X-ray diffraction
1.8Å resolution

Structure of an ADP ribosylation factor from Entamoeba histolytica HM-1:IMSS bound to Mg-GDP

Released:
DOI: 10.2210/pdb4y0v/pdb
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Source organism: Entamoeba histolytica
Primary publication:
Structure of an ADP-ribosylation factor, ARF1, from Entamoeba histolytica bound to Mg(2+)-GDP.
Serbzhinskiy DA, Clifton MC, Sankaran B, Staker BL, Edwards TE, Myler PJ
Acta Crystallogr F Struct Biol Commun 71 594-9 (2015)
PMID: 25945714

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylation factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 20.21 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: C4LXL1 (Residues: 1-174; Coverage: 100%)
Gene names: EHI_073470, EHI_137720
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: C2
Unit cell:
a: 123.54Å b: 40.66Å c: 78.95Å
α: 90° β: 97.45° γ: 90°
R-values:
R R work R free
0.225 0.223 0.272
Expression system: Escherichia coli

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Citations

18 citation in other articles

Missense-depleted regions in population exomes implicate ras superfamily nucleotide-binding protein alteration in patients with brain malformation.
Ge et al. (2016)
17 more

1 mention without citation

Structure of an ADP-ribosylation factor, ARF1, from Entamoeba histolytica bound to Mg(2+)-GDP.
Serbzhinskiy et al. (2015)

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