› 4y1k

X-ray diffraction
3.8Å resolution

PALMITOYLATED OPRM OUTER MEMBRANE FACTOR

Released:
DOI: 10.2210/pdb4y1k/pdb
PDB entry 4y1k coloured by chain, front view.
PDB entry 4y1k coloured by chain, side view.
PDB entry 4y1k coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
New OprM structure highlighting the nature of the N-terminal anchor.
Monlezun L, Phan G, Benabdelhak H, Lascombe MB, Enguéné VY, Picard M, Broutin I
OpenAccess logo Front Microbiol 6 667 (2015)
PMID: 26191054

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175945 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein OprM Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 474 amino acids
Theoretical weight: 51.74 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51487 (Residues: 18-485; Coverage: 100%)
Gene names: PA0427, oprK, oprM
Sequence domains: Outer membrane efflux protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PLM 3 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 152.642Å b: 87.858Å c: 355.942Å
α: 90° β: 98.94° γ: 90°
R-values:
R R work R free
0.299 0.297 0.346
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
2 more

2 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
1 more