› 4y91

X-ray diffraction
2.66Å resolution

Crystal Structure of a Thermotoga maritima Hfq homolog

Released:
DOI: 10.2210/pdb4y91/pdb
PDB entry 4y91 coloured by chain, front view.
PDB entry 4y91 coloured by chain, side view.
PDB entry 4y91 coloured by chain, top view.
Source organisms:
Entry authors: Randolph PS, Patterson J, Mura C

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-578220 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-binding protein Hfq Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 95 amino acids
Theoretical weight: 10.81 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WYZ6 (Residues: 1-92; Coverage: 100%)
Gene names: TM_0526, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.
RNA (5'-R(P*UP*UP*UP*UP*UP*U)-3') Chains: N, O
Molecule details ›
Chains: N, O
Length: 6 nucleotides
Theoretical weight: 1.79 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 39.08Å b: 133.5Å c: 206.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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