› 4ywk

X-ray diffraction
1.55Å resolution

Pyrococcus furiosus MCM N-terminal domain with Zinc-binding subdomain B deleted

Released:
DOI: 10.2210/pdb4ywk/pdb
PDB entry 4ywk coloured by chain, front view.
PDB entry 4ywk coloured by chain, side view.
PDB entry 4ywk coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
MCM ring hexamerization is a prerequisite for DNA-binding.
Froelich CA, Nourse A, Enemark EJ
OpenAccess logo Nucleic Acids Res 43 9553-63 (2015)
PMID: 26365238

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-186462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA helicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 206 amino acids
Theoretical weight: 23.68 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U3I4 (Residues: 2-256; Coverage: 19%)
Gene name: PF0482
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 41.958Å b: 50.964Å c: 52.88Å
α: 96.54° β: 94.77° γ: 95.45°
R-values:
R R work R free
0.219 0.217 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Fundamental Characteristics of AAA+ Protein Family Structure and Function.
Miller et al. (2016)
1 more