4z59

X-ray diffraction
2.3Å resolution

HipB-O4 20mer complex

Released:
Entry authors: Min J, Brennan RG, Schumacher MA

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118357 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Antitoxin HipB Chain: A
Molecule details ›
Chain: A
Length: 71 amino acids
Theoretical weight: 8.06 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23873 (Residues: 4-74; Coverage: 81%)
Gene names: JW1501, b1508, hipB
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*TP*TP*AP*TP*CP*CP*GP*CP*GP*AP*TP*CP*GP*CP*GP*GP*AP*TP*AP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P4132
Unit cell:
a: 127.37Å b: 127.37Å c: 127.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.152 0.182
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided