› 4zff

X-ray diffraction
2.75Å resolution

Dual-acting Fab 5A12 in complex with VEGF

Released:
DOI: 10.2210/pdb4zff/pdb
PDB entry 4zff coloured by chain, front view.
PDB entry 4zff coloured by chain, side view.
PDB entry 4zff coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Deep Sequencing-guided Design of a High Affinity Dual Specificity Antibody to Target Two Angiogenic Factors in Neovascular Age-related Macular Degeneration.
Koenig P, Lee CV, Sanowar S, Wu P, Stinson J, Harris SF, Fuh G
J Biol Chem 290 21773-86 (2015)
PMID: 26088137

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210648 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fragment antigen binding (Fab) 5A12 Heavy chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 228 amino acids
Theoretical weight: 24.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fragment antigen binding (Fab) 5A12 Light chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 215 amino acids
Theoretical weight: 23.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
N-VEGF Chains: C, D
Molecule details ›
Chains: C, D
Length: 99 amino acids
Theoretical weight: 11.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15692 (Residues: 217-315; Coverage: 25%)
Gene names: VEGF, VEGFA
Sequence domains: PDGF/VEGF domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21212
Unit cell:
a: 87.406Å b: 313.892Å c: 51.127Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.238
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Functional assays for transcription mechanisms in high-throughput.
Qiu et al. (2019)
3 more

3 mentions without citation

Deep sequencing methods for protein engineering and design.
Wrenbeck et al. (2017)
2 more