› 4zkw

X-ray diffraction
1.8Å resolution

Crystal structure of the PmFTN variant E44Q soaked in iron (45 min)

Released:
DOI: 10.2210/pdb4zkw/pdb
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Source organism: Pseudo-nitzschia multiseries
Primary publication:
A Diatom Ferritin Optimized for Iron Oxidation but Not Iron Storage.
Pfaffen S, Bradley JM, Abdulqadir R, Firme MR, Moore GR, Le Brun NE, Murphy MEP
OpenAccess logo J Biol Chem 290 28416-28427 (2015)
PMID: 26396187

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 169 amino acids
Theoretical weight: 18.97 KDa
Source organism: Pseudo-nitzschia multiseries
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B6DMH6 (Residues: 63-230; Coverage: 80%)
Gene name: FTN
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

1 bound ligand:
Ligand FE 16 x FE
1 modified residue:
Ligand YCM 8 x YCM

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P23
Unit cell:
a: 174.92Å b: 174.92Å c: 174.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.205
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Ferritin, cellular iron storage and regulation.
Arosio et al. (2017)
4 more

2 mentions without citation

Vaccine technologies: From whole organisms to rationally designed protein assemblies.
Karch et al. (2016)
1 more

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