Function and Biology Details
Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
RNA polymerase B3 3Dpol (preferred)
PDBe Complex ID:
PDB-CPX-178362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-dependent RNA polymerase
Molecule details ›
Chain: A
Length: 462 amino acids
Theoretical weight: 52.44 KDa
Source organism: Coxsackievirus B3
Expression system: Escherichia coli BL21(DE3)
UniProt:
Structure domains:
Length: 462 amino acids
Theoretical weight: 52.44 KDa
Source organism: Coxsackievirus B3
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q5UEA2 (Residues: 1724-2185; Coverage: 21%)
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ALS BEAMLINE 4.2.2
Spacegroup:
P43212
Expression system: Escherichia coli BL21(DE3)
