› 4zsz

X-ray diffraction
4.25Å resolution

Structure of a fusion protein with a helix linker, 2ARH-3-3KAW-3.0

Released:
DOI: 10.2210/pdb4zsz/pdb
PDB entry 4zsz coloured by chain, front view.
PDB entry 4zsz coloured by chain, side view.
PDB entry 4zsz coloured by chain, top view.
Source organisms:
Primary publication:
On the predictability of the orientation of protein domains joined by a spanning alpha-helical linker.
Lai YT, Jiang L, Chen W, Yeates TO
Protein Eng Des Sel 28 491-9 (2015)
PMID: 26243886

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF1122 domain-containing protein; Four-helix bundle copper-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 34.34 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67778 (Residues: 1-196; Coverage: 98%)
  • Canonical: Q9I208 (Residues: 35-130; Coverage: 73%)
Gene names: PA2107, aq_1966
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P3121
Unit cell:
a: 112.93Å b: 112.93Å c: 150.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.266 0.264 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Geometric Principles for Designing Highly Symmetric Self-Assembling Protein Nanomaterials.
Yeates TO. (2017)
5 more