› 4zyp

X-ray diffraction
5.5Å resolution

Crystal Structure of Motavizumab and Quaternary-Specific RSV-Neutralizing Human Antibody AM14 in Complex with Prefusion RSV F Glycoprotein

Released:
DOI: 10.2210/pdb4zyp/pdb
PDB entry 4zyp coloured by chain, front view.
PDB entry 4zyp coloured by chain, side view.
PDB entry 4zyp coloured by chain, top view.
Source organisms:
Primary publication:
Characterization of a Prefusion-Specific Antibody That Recognizes a Quaternary, Cleavage-Dependent Epitope on the RSV Fusion Glycoprotein.
Gilman MS, Moin SM, Mas V, Chen M, Patel NK, Kramer K, Zhu Q, Kabeche SC, Kumar A, Palomo C, Beaumont T, Baxa U, Ulbrandt ND, Melero JA, Graham BS, McLellan JS
OpenAccess logo PLoS Pathog 11 e1005035 (2015)
PMID: 26161532

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229694 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Fibritin C-terminal domain-containing protein; Fusion glycoprotein F0 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 498 amino acids
Theoretical weight: 55.12 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P03420 (Residues: 26-513; Coverage: 84%)
  • Canonical: D9IEJ2 (Residues: 458-484; Coverage: 6%)
Gene names: F, T4Tp161, wac
Sequence domains: Fusion glycoprotein F0
Motavizumab antibody Fab heavy chain Chains: J, K, N
Molecule details ›
Chains: J, K, N
Length: 225 amino acids
Theoretical weight: 24.28 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Motavizumab antibody light chain Chains: L, M, O
Molecule details ›
Chains: L, M, O
Length: 213 amino acids
Theoretical weight: 23.15 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
AM14 antibody Fab heavy chain Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 227 amino acids
Theoretical weight: 24.37 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
AM14 antibody light chain Chains: E, G, I
Molecule details ›
Chains: E, G, I
Length: 215 amino acids
Theoretical weight: 23.62 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 114.38Å b: 210.29Å c: 118.2Å
α: 90° β: 100.46° γ: 90°
R-values:
R R work R free
0.214 0.21 0.277
Expression system: Homo sapiens

Quick links

Citations

14 review citations

Heparan Sulfate Proteoglycans and Viral Attachment: True Receptors or Adaptation Bias?
Cagno et al. (2019)
13 more

13 mentions without citation

Approved Antiviral Drugs over the Past 50 Years.
De Clercq et al. (2016)
12 more