› 5a1j

X-ray diffraction
1.6Å resolution

Periplasmic Binding Protein CeuE in complex with ferric 4-LICAM

Released:
DOI: 10.2210/pdb5a1j/pdb
PDB entry 5a1j coloured by chain, front view.
PDB entry 5a1j coloured by chain, side view.
PDB entry 5a1j coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Interactions of a periplasmic binding protein with a tetradentate siderophore mimic.
Raines DJ, Moroz OV, Wilson KS, Duhme-Klair AK
Angew Chem Int Ed Engl 52 4595-8 (2013)
PMID: 23512642

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 288 amino acids
Theoretical weight: 31.86 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0P8Q4 (Residues: 44-330; Coverage: 93%)
Gene names: Cj1355, ceuE
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand LCM 1 x LCM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 60.42Å b: 66.89Å c: 67.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.264 0.312
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Artificial imine reductases: developments and future directions.
Booth et al. (2020)
10 other articles

4 mentions without citation

Iron Acquisition by Bacterial Pathogens: Beyond Tris-Catecholate Complexes.
Zhang et al. (2020)
3 more