› 5a4a

X-ray diffraction
1.7Å resolution

Crystal structure of the OSK domain of Drosophila Oskar

Released:
DOI: 10.2210/pdb5a4a/pdb
PDB entry 5a4a coloured by chain, front view.
PDB entry 5a4a coloured by chain, side view.
PDB entry 5a4a coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The Crystal Structure of the Drosophila Germline Inducer Oskar Identifies Two Domains with Distinct Vasa Helicase- and RNA-Binding Activities.
Jeske M, Bordi M, Glatt S, Müller S, Rybin V, Müller CW, Ephrussi A
Cell Rep 12 587-98 (2015)
PMID: 26190108

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150398 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maternal effect protein oskar Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 24.17 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P25158 (Residues: 401-606; Coverage: 34%)
Gene names: CG10901, osk
Sequence domains: OSK domain
Structure domains: SGNH hydrolase

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3121
Unit cell:
a: 68.43Å b: 68.43Å c: 101.07Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.166 0.194
Expression system: Escherichia coli

Quick links

Citations

15 review citations

piRNA Biogenesis in Drosophila melanogaster.
Huang et al. (2017)
14 more

3 mentions without citation

Subtype specific differences in NS5A domain II reveals involvement of proline at position 310 in cyclosporine susceptibility of hepatitis C virus.
Ansari et al. (2012)
2 more