› 5abj

X-ray diffraction
2.75Å resolution

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 240-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125106 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
VP1 Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 33.08 KDa
Source organism: Coxsackievirus A16
Expression system: Chlorocebus aethiops
UniProt:
  • Canonical: I3W9E1 (Residues: 566-862; Coverage: 14%)
Sequence domains: Picornavirus coat protein
Structure domains: Jelly Rolls
VP2 Chain: B
Molecule details ›
Chain: B
Length: 254 amino acids
Theoretical weight: 27.56 KDa
Source organism: Coxsackievirus A16
Expression system: Chlorocebus aethiops
UniProt:
  • Canonical: I3W9E1 (Residues: 70-323; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
VP3 Chain: C
Molecule details ›
Chain: C
Length: 242 amino acids
Theoretical weight: 26.65 KDa
Source organism: Coxsackievirus A16
Expression system: Chlorocebus aethiops
UniProt:
  • Canonical: I3W9E1 (Residues: 324-565; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
VP4 Chain: D
Molecule details ›
Chain: D
Length: 69 amino acids
Theoretical weight: 7.55 KDa
Source organism: Coxsackievirus A16
Expression system: Chlorocebus aethiops
UniProt:
  • Canonical: I3W9E1 (Residues: 1-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)
Structure domains: Rhinovirus 14, subunit 4

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand YM2 1 x YM2
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P41212
Unit cell:
a: 491.6Å b: 491.6Å c: 709.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.305 0.305 0.307
Expression system: Chlorocebus aethiops

Quick links

Citations

1 review citation

Antivirals blocking entry of enteroviruses and therapeutic potential.
Anasir et al. (2021)
9 other articles

1 mention without citation

Potent antiviral agents fail to elicit genetically-stable resistance mutations in either enterovirus 71 or Coxsackievirus A16.
Kelly et al. (2015)