› 5abv

X-ray diffraction
2.13Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155769 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E1 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 184 amino acids
Theoretical weight: 21.25 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48598 (Residues: 80-259; Coverage: 70%)
Gene names: CG4035, Eif4e, eIF-4E, eIF4E1
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
Eukaryotic translation initiation factor 4E-binding protein Mextli Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 70 amino acids
Theoretical weight: 8.23 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VR35 (Residues: 577-640; Coverage: 10%)
Gene names: CG2950, mxt

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 65.9Å b: 82.02Å c: 84.73Å
α: 90° β: 90.13° γ: 90°
R-values:
R R work R free
0.194 0.192 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

A Series of Tubes: The C. elegans Excretory Canal Cell as a Model for Tubule Development.
Buechner et al. (2020)
11 other articles

2 mentions without citation

Control of the eIF4E activity: structural insights and pharmacological implications.
Romagnoli et al. (2021)
1 more