› 5adw

X-ray diffraction
1.9Å resolution

The Periplasmic Binding Protein CeuE of Campylobacter jejuni preferentially binds the iron(III) complex of the Linear Dimer Component of Enterobactin

Released:
DOI: 10.2210/pdb5adw/pdb
PDB entry 5adw coloured by chain, front view.
PDB entry 5adw coloured by chain, side view.
PDB entry 5adw coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Bacteria in an intense competition for iron: Key component of the Campylobacter jejuni iron uptake system scavenges enterobactin hydrolysis product.
Raines DJ, Moroz OV, Blagova EV, Turkenburg JP, Wilson KS, Duhme-Klair AK
Proc Natl Acad Sci U S A 113 5850-5 (2016)
PMID: 27162326

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 287 amino acids
Theoretical weight: 31.73 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0P8Q4 (Residues: 44-330; Coverage: 93%)
Gene names: Cj1355, ceuE
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

3 bound ligands:
Ligand FE 3 x FE
Ligand EHS 2 x EHS
Ligand DMS 3 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 58.461Å b: 63.021Å c: 67.011Å
α: 83.36° β: 76.57° γ: 78.24°
R-values:
R R work R free
0.227 0.225 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Chemical and Biological Aspects of Nutritional Immunity-Perspectives for New Anti-Infectives that Target Iron Uptake Systems.
Bilitewski et al. (2017)
3 more

3 mentions without citation

SuFEx-enabled, agnostic discovery of covalent inhibitors of human neutrophil elastase.
Zheng et al. (2019)
2 more