› 5amc

X-ray diffraction
1.65Å resolution

Crystal structure of the Angiotensin-1 converting enzyme N-domain in complex with amyloid-beta fluorogenic fragment 4-10

Released:
DOI: 10.2210/pdb5amc/pdb
PDB entry 5amc coloured by chain, front view.
PDB entry 5amc coloured by chain, side view.
PDB entry 5amc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Kinetic and structural characterization of amyloid-β peptide hydrolysis by human angiotensin-1-converting enzyme.
Larmuth KM, Masuyer G, Douglas RG, Schwager SL, Acharya KR, Sturrock ED
OpenAccess logo FEBS J 283 1060-76 (2016)
PMID: 26748546

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146325 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (6 distinct):
Angiotensin-converting enzyme, soluble form Chain: A
Molecule details ›
Chain: A
Length: 629 amino acids
Theoretical weight: 72.61 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P12821 (Residues: 30-658; Coverage: 49%)
Gene names: ACE, DCP, DCP1
Sequence domains: Angiotensin-converting enzyme
Angiotensin-converting enzyme, soluble form Chain: B
Molecule details ›
Chain: B
Length: 629 amino acids
Theoretical weight: 72.61 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P12821 (Residues: 30-658; Coverage: 49%)
Gene names: ACE, DCP, DCP1
Sequence domains: Angiotensin-converting enzyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
7 bound ligands:
Ligand GLY 2 x GLY
Ligand NIY 2 x NIY
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand PEG 6 x PEG
Ligand PG4 1 x PG4
Ligand P6G 1 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 73.029Å b: 76.47Å c: 83.158Å
α: 88.78° β: 64.25° γ: 75.64°
R-values:
R R work R free
0.209 0.208 0.242
Expression system: Cricetulus griseus

Quick links

Citations

2 review citations

ACE2 and ACE: structure-based insights into mechanism, regulation and receptor recognition by SARS-CoV.
Lubbe et al. (2020)
1 more

5 mentions without citation

Cell-Autonomous Regulation of Astrocyte Activation by the Circadian Clock Protein BMAL1.
Lananna et al. (2018)
4 more