5amq

X-ray diffraction
3Å resolution

Structure of the La Crosse Bunyavirus polymerase in complex with the 3' and 5' viral RNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-107608 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct RNA molecules
Macromolecules (4 distinct):
RNA-directed RNA polymerase L Chain: A
Molecule details ›
Chain: A
Length: 2263 amino acids
Theoretical weight: 263.37 KDa
Source organism: La Crosse virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A5HC98 (Residues: 1-2263; Coverage: 100%)
Gene name: L
Sequence domains:
RNA Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 5.06 KDa
Source organism: La Crosse virus
Expression system: Trichoplusia ni
RNA Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3.2 KDa
Source organism: La Crosse virus
Expression system: Trichoplusia ni
RNA Chain: c
Molecule details ›
Chain: c
Length: 8 nucleotides
Theoretical weight: 2.51 KDa
Source organism: La Crosse virus
Expression system: Trichoplusia ni

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 103Å b: 141.1Å c: 165.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.252
Expression system: Trichoplusia ni