5aq9

X-ray diffraction
1.86Å resolution

DARPin-based Crystallization Chaperones exploit Molecular Geometry as a Screening Dimension in Protein Crystallography

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165444 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
OFF7_DB08V4 Chains: A, C
Molecule details ›
Chains: A, C
Length: 422 amino acids
Theoretical weight: 45.68 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12
Structure domains:
Maltose/maltodextrin-binding periplasmic protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 395 amino acids
Theoretical weight: 43.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0AEX9 (Residues: 33-392; Coverage: 97%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 41.48Å b: 191.12Å c: 219.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.204
Expression system: Escherichia coli K-12