5axl

X-ray diffraction
3Å resolution

Crystal structure of Thg1 like protein (TLP) with GTP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-103327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNAHis guanylyltransferase catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 29.33 KDa
Source organism: Methanosarcina acetivorans
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A1C7D1G9 (Residues: 1-251; Coverage: 100%)
Sequence domains: tRNAHis guanylyltransferase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2221
Unit cell:
a: 103.125Å b: 115.693Å c: 144.882Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.253
Expression system: Escherichia coli BL21