› 5b02

X-ray diffraction
2.21Å resolution

Structure of the prenyltransferase MoeN5 with a fusion protein tag of Sso7d

Released:
DOI: 10.2210/pdb5b02/pdb
PDB entry 5b02 coloured by chain, front view.
PDB entry 5b02 coloured by chain, side view.
PDB entry 5b02 coloured by chain, top view.
Source organisms:
Primary publication:
Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5.
Zhang L, Chen CC, Ko TP, Huang JW, Zheng Y, Liu W, Wang I, Malwal SR, Feng X, Wang K, Huang CH, Hsu ST, Wang AH, Oldfield E, Guo RT
Angew Chem Int Ed Engl 55 4716-20 (2016)
PMID: 26954060

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100015 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding protein 7d; MoeN5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 343 amino acids
Theoretical weight: 37.58 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: A0A010 (Residues: 1-260; Coverage: 100%)
  • Canonical: P39476 (Residues: 1-64; Coverage: 100%)
Gene names: SSO10610, moeN5, sso7d, sso7d-1
Sequence domains: 7kD DNA-binding domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C222
Unit cell:
a: 137.879Å b: 217.441Å c: 104.637Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.218
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Bacterial terpenome.
Rudolf et al. (2021)
1 more

1 mention without citation

Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5.
Zhang et al. (2016)