› 5b1o

X-ray diffraction
2.3Å resolution

DHp domain structure of EnvZ P248A mutant

Released:
DOI: 10.2210/pdb5b1o/pdb
PDB entry 5b1o coloured by chain, front view.
PDB entry 5b1o coloured by chain, side view.
PDB entry 5b1o coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Angucycline antibiotic waldiomycin recognizes common structural motif conserved in bacterial histidine kinases.
Eguchi Y, Okajima T, Tochio N, Inukai Y, Shimizu R, Ueda S, Shinya S, Kigawa T, Fukamizo T, Igarashi M, Utsumi R
J Antibiot (Tokyo) 70 251-258 (2017)
PMID: 27999439

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensor histidine kinase EnvZ Chains: A, B
Molecule details ›
Chains: A, B
Length: 69 amino acids
Theoretical weight: 7.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEJ4 (Residues: 223-289; Coverage: 15%)
Gene names: JW3367, b3404, envZ, ompB, perA, tpo
Sequence domains: His Kinase A (phospho-acceptor) domain
Structure domains: Helix Hairpins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P3121
Unit cell:
a: 35.761Å b: 35.761Å c: 191.248Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.235 0.272
Expression system: Escherichia coli

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Citations

7 review citations

β-Lactams against the Fortress of the Gram-Positive Staphylococcus aureus Bacterium.
Fisher et al. (2021)
6 more