› 5b7f

X-ray diffraction
1.45Å resolution

Structure of CueO - the signal peptide was truncated by HRV3C protease

Released:
DOI: 10.2210/pdb5b7f/pdb
PDB entry 5b7f coloured by chain, front view.
PDB entry 5b7f coloured by chain, side view.
PDB entry 5b7f coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Biochemical, spectroscopic and X-ray structural analysis of deuterated multicopper oxidase CueO prepared from a new expression construct for neutron crystallography.
Akter M, Inoue C, Komori H, Matsuda N, Sakurai T, Kataoka K, Higuchi Y, Shibata N
Acta Crystallogr F Struct Biol Commun 72 788-794 (2016)
PMID: 27710945

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multicopper oxidase CueO Chain: A
Molecule details ›
Chain: A
Length: 492 amino acids
Theoretical weight: 53.75 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P36649 (Residues: 29-516; Coverage: 100%)
Gene names: JW0119, b0123, cueO, yacK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand CU 4 x CU
Ligand EDO 1 x EDO
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 49.513Å b: 88.786Å c: 53.951Å
α: 90° β: 94.24° γ: 90°
R-values:
R R work R free
0.153 0.153 0.181
Expression system: Escherichia coli

Quick links

Citations

2 mentions without citation

Modelling and Recognition of Protein Contact Networks by Multiple Kernel Learning and Dissimilarity Representations.
Martino et al. (2020)
1 more