› 5b7m

X-ray diffraction
1.8Å resolution

Structure of perdeuterated CueO - the signal peptide was truncated by HRV3C protease

Released:
DOI: 10.2210/pdb5b7m/pdb
PDB entry 5b7m coloured by chain, front view.
PDB entry 5b7m coloured by chain, side view.
PDB entry 5b7m coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Biochemical, spectroscopic and X-ray structural analysis of deuterated multicopper oxidase CueO prepared from a new expression construct for neutron crystallography.
Akter M, Inoue C, Komori H, Matsuda N, Sakurai T, Kataoka K, Higuchi Y, Shibata N
Acta Crystallogr F Struct Biol Commun 72 788-794 (2016)
PMID: 27710945

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multicopper oxidase CueO Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 492 amino acids
Theoretical weight: 53.75 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P36649 (Residues: 29-516; Coverage: 100%)
Gene names: JW0119, b0123, cueO, yacK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 12 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P212121
Unit cell:
a: 49.911Å b: 106.915Å c: 262.892Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.204
Expression system: Escherichia coli

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Citations

1 mention without citation

Biochemical, spectroscopic and X-ray structural analysis of deuterated multicopper oxidase CueO prepared from a new expression construct for neutron crystallography.
Akter et al. (2016)