› 5bmz

X-ray diffraction
3Å resolution

Crystal Structure of Putative MarR Family Transcriptional Regulator HcaR from Acinetobacter sp. ADP complexed with 24mer DNA.

Released:
DOI: 10.2210/pdb5bmz/pdb
PDB entry 5bmz coloured by chain, front view.
PDB entry 5bmz coloured by chain, side view.
PDB entry 5bmz coloured by chain, top view.
Source organism: Acinetobacter baylyi ADP1
Entry authors: Kim Y, Joachimiak G, Biglow L, Cobb G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118428 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH marR-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 162 amino acids
Theoretical weight: 18.37 KDa
Source organism: Acinetobacter baylyi ADP1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7X0D9 (Residues: 1-159; Coverage: 100%)
Gene names: ACIAD1728, hcaR
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(P*GP*AP*AP*TP*AP*TP*CP*AP*GP*TP*TP*AP*AP*AP*CP*TP*GP*AP*TP*AP*TP*TP*C)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 24 nucleotides
Theoretical weight: 7.35 KDa
Source organism: Acinetobacter baylyi ADP1
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P61
Unit cell:
a: 161.965Å b: 161.965Å c: 73.827Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.252 0.294
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links