› 5bqf

X-ray diffraction
1.45Å resolution

Probable 2-hydroxyacid dehydrogenase from Rhizobium etli CFN 42 in complex with NADP, HEPES and L(+)-tartaric acid

Released:
DOI: 10.2210/pdb5bqf/pdb
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PDB entry 5bqf coloured by chain, front view.
PDB entry 5bqf coloured by chain, side view.
PDB entry 5bqf coloured by chain, top view.
Source organism: Rhizobium etli CFN 42
Entry authors: Langner KM, Shabalin IG, Handing KB, Gasiorowska OA, Stead M, Hillerich BS, Chowdhury S, Hammonds J, Zimmerman MD, Al Obaidi N, Bonanno J, Seidel R, Almo SC, Minor W, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 35.79 KDa
Source organism: Rhizobium etli CFN 42
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2KDT2 (Residues: 1-319; Coverage: 100%)
Gene name: RHE_CH00179
Sequence domains: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

6 bound ligands:
Ligand EPE 1 x EPE
Ligand PEG 1 x PEG
Ligand NAP 1 x NAP
Ligand TLA 1 x TLA
Ligand NA 1 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 65.692Å b: 65.692Å c: 151.406Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.138 0.165
Expression system: Escherichia coli

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