› 5bt4

X-ray diffraction
1.5Å resolution

Crystal structure of BRD4 first bromodomain in complex with SGC-CBP30 chemical probe

Released:
DOI: 10.2210/pdb5bt4/pdb
PDB entry 5bt4 coloured by chain, front view.
PDB entry 5bt4 coloured by chain, side view.
PDB entry 5bt4 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Tallant C, Hay D, Krojer T, Nunez-Alonso G, Picaud S, Newman JA, Fedorov O, von Delft F, Arrowsmith CH, Edwards AM, Bountra C, Brennan PE, Knapp S, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bromodomain-containing protein 4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 127 amino acids
Theoretical weight: 15.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60885 (Residues: 44-168; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

2 bound ligands:
Ligand 2LO 3 x 2LO
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 41.923Å b: 91.801Å c: 113.761Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.214
Expression system: Escherichia coli BL21(DE3)

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