› 5by6

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 307 amino acids
Theoretical weight: 35.54 KDa
Source organism: Trichinella spiralis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NDD3 (Residues: 1-307; Coverage: 100%)
Gene name: ts
Sequence domains: Thymidylate synthase
Structure domains: Thymidylate synthase/dCMP hydroxymethylase domain

Ligands and Environments

3 bound ligands:
Ligand UMP 4 x UMP
Ligand DTT 1 x DTT
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P1
Unit cell:
a: 51.695Å b: 65.914Å c: 96.511Å
α: 85.31° β: 85.33° γ: 67.12°
R-values:
R R work R free
0.167 0.165 0.22
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Alvaxanthone, a Thymidylate Synthase Inhibitor with Nematocidal and Tumoricidal Activities.
Maj et al. (2020)

1 mention without citation

Molecular Mechanism of Thymidylate Synthase Inhibition by N4-Hydroxy-dCMP in View of Spectrophotometric and Crystallographic Studies.
Maj et al. (2021)