5c0c

X-ray diffraction
1.97Å resolution

1E6 TCR in complex with HLA-A02 carrying RQFGPDWIVA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-100928 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 277 amino acids
Theoretical weight: 32.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, G
Molecule details ›
Chains: B, G
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Marker peptide Chains: C, H
Molecule details ›
Chains: C, H
Length: 10 amino acids
Theoretical weight: 1.19 KDa
Source organism: Homo sapiens
Expression system: Not provided
T cell receptor alpha chain constant; T cell receptor alpha variable 12-3 Chains: D, I
Molecule details ›
Chains: D, I
Length: 200 amino acids
Theoretical weight: 22.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B4J271 (Residues: 24-113; Coverage: 97%)
  • Canonical: P01848 (Residues: 1-89; Coverage: 64%)
Gene names: TCRA, TRAC, TRAV12-3
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 2; T cell receptor beta variable 12-4 Chains: E, J
Molecule details ›
Chains: E, J
Length: 247 amino acids
Theoretical weight: 28.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B4J2E0 (Residues: 20-115; Coverage: 100%)
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
Gene names: TCRBC2, TRBC2, TRBV12-4
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 43.92Å b: 100.37Å c: 122.73Å
α: 96.9° β: 98.02° γ: 96.07°
R-values:
R R work R free
0.19 0.188 0.227
Expression systems:
  • Escherichia coli
  • Not provided