› 5c0u

X-ray diffraction
1.87Å resolution

Function and Biology Details

Reaction catalysed: 
An alkylmercury + H(+) = an alkane + Hg(2+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160120 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkylmercury lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77072 (Residues: 1-212; Coverage: 100%)
Gene name: merB
Sequence domains:
Structure domains: Beta-Lactamase

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand BR 1 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 37.903Å b: 88.958Å c: 52.005Å
α: 90° β: 100.64° γ: 90°
R-values:
R R work R free
0.151 0.147 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Demethylation-The Other Side of the Mercury Methylation Coin: A Critical Review.
Barkay et al. (2022)
6 other articles