› 5cd7

X-ray diffraction
2.5Å resolution

Crystal structure of the NTD L199M of Drosophila Oskar protein

Released:
DOI: 10.2210/pdb5cd7/pdb
PDB entry 5cd7 coloured by chain, front view.
PDB entry 5cd7 coloured by chain, side view.
PDB entry 5cd7 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure of Drosophila Oskar reveals a novel RNA binding protein.
Yang N, Yu Z, Hu M, Wang M, Lehmann R, Xu RM
Proc Natl Acad Sci U S A 112 11541-6 (2015)
PMID: 26324911

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150399 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maternal effect protein oskar Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 77 amino acids
Theoretical weight: 8.62 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P25158 (Residues: 150-224; Coverage: 12%)
Gene names: CG10901, osk
Sequence domains: OST-HTH/LOTUS domain

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 62.962Å b: 38.307Å c: 102.13Å
α: 90° β: 95.54° γ: 90°
R-values:
R R work R free
0.217 0.214 0.27
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Germ Plasm Biogenesis--An Oskar-Centric Perspective.
Lehmann R. (2016)
8 more

1 mention without citation

Structure of Drosophila Oskar reveals a novel RNA binding protein.
Yang et al. (2015)