› 5cxd

X-ray diffraction
1.75Å resolution

1.75 Angstrom resolution crystal structure of the apo-form acyl-carrier-protein synthase (AcpS) (acpS; purification tag off) from Staphylococcus aureus subsp. aureus COL in the I4 space group

Released:
DOI: 10.2210/pdb5cxd/pdb
PDB entry 5cxd coloured by chain, front view.
PDB entry 5cxd coloured by chain, side view.
PDB entry 5cxd coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus COL
Entry authors: Halavaty AS, Minasov G, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
CoA-(4'-phosphopantetheine) + an apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + an [acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177182 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holo-[acyl-carrier-protein] synthase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 122 amino acids
Theoretical weight: 13.9 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5HED0 (Residues: 1-119; Coverage: 100%)
Gene names: SACOL2061, acpS
Sequence domains: 4'-phosphopantetheinyl transferase superfamily
Structure domains: 4'-phosphopantetheinyl transferase domain

Ligands and Environments

3 bound ligands:
Ligand CL 12 x CL
Ligand NI 1 x NI
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: I4
Unit cell:
a: 103.117Å b: 103.117Å c: 71.189Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.203
Expression system: Escherichia coli BL21(DE3)

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