› 5d8j

X-ray diffraction
3Å resolution

Development of a therapeutic monoclonal antibody targeting secreted aP2 to treat type 2 diabetes.

Released:
DOI: 10.2210/pdb5d8j/pdb
PDB entry 5d8j coloured by chain, front view.
PDB entry 5d8j coloured by chain, side view.
PDB entry 5d8j coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Development of a therapeutic monoclonal antibody that targets secreted fatty acid-binding protein aP2 to treat type 2 diabetes.
Burak MF, Inouye KE, White A, Lee A, Tuncman G, Calay ES, Sekiya M, Tirosh A, Eguchi K, Birrane G, Lightwood D, Howells L, Odede G, Hailu H, West S, Garlish R, Neale H, Doyle C, Moore A, Hotamisligil GS
Sci Transl Med 7 319ra205 (2015)
PMID: 26702093

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208975 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fatty acid-binding protein, adipocyte Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 14.82 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04117 (Residues: 1-132; Coverage: 100%)
Gene names: Ap2, Fabp4
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin
HA3 Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.73 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
HA3 Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.52 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 71.499Å b: 66.04Å c: 75.678Å
α: 90° β: 111.67° γ: 90°
R-values:
R R work R free
0.208 0.205 0.269
Expression systems:
  • Escherichia coli
  • Cricetulus griseus

Quick links

Citations

14 review citations

The endocrine function of adipose tissues in health and cardiometabolic disease.
Scheja et al. (2019)
13 more

2 mentions without citation

Generation of Long-Lived Bone Marrow Plasma Cells Secreting Antibodies Specific for the HIV-1 gp41 Membrane-Proximal External Region in the Absence of Polyreactivity.
Donius et al. (2016)
1 more