› 5dgc

X-ray diffraction
1.94Å resolution

Reaction of phosphorylated CheY with imidazole 2 of 3

Released:
DOI: 10.2210/pdb5dgc/pdb
PDB entry 5dgc coloured by chain, front view.
PDB entry 5dgc coloured by chain, side view.
PDB entry 5dgc coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Imidazole as a Small Molecule Analogue in Two-Component Signal Transduction.
Page SC, Silversmith RE, Collins EJ, Bourret RB
Biochemistry 54 7248-60 (2015)
PMID: 26569142

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chemotaxis protein CheY Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 14.01 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE68 (Residues: 2-129; Coverage: 99%)
Gene names: ECs2592, Z2936, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

5 bound ligands:
Ligand BEF 2 x BEF
Ligand MN 2 x MN
Ligand IMD 6 x IMD
Ligand CL 1 x CL
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 53.49Å b: 53.58Å c: 161.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.189
Expression system: Escherichia coli

Quick links

Citations

2 review citations

A Radical Reimagining of Fungal Two-Component Regulatory Systems.
Bourret et al. (2021)
1 more

1 mention without citation

Imidazole as a Small Molecule Analogue in Two-Component Signal Transduction.
Page et al. (2015)