› 5dky

X-ray diffraction
1.6Å resolution

Crystal structure of glucosidase II alpha subunit (DNJ-bound from)

Released:
DOI: 10.2210/pdb5dky/pdb
PDB entry 5dky coloured by chain, front view.
PDB entry 5dky coloured by chain, side view.
PDB entry 5dky coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Structural basis for two-step glucose trimming by glucosidase II involved in ER glycoprotein quality control.
Satoh T, Toshimori T, Yan G, Yamaguchi T, Kato K
OpenAccess logo Sci Rep 6 20575 (2016)
PMID: 26847925

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
alpha-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 951 amino acids
Theoretical weight: 108.5 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0SG42 (Residues: 31-977; Coverage: 100%)
Gene name: CTHT_0064960
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NOJ 1 x NOJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: R32
Unit cell:
a: 189.523Å b: 189.523Å c: 157.784Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.155 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Iminosugar antivirals: the therapeutic sweet spot.
Alonzi et al. (2017)
1 more

1 mention without citation

Structural basis for two-step glucose trimming by glucosidase II involved in ER glycoprotein quality control.
Satoh et al. (2016)