› 5dow

X-ray diffraction
1.7Å resolution

Solution of the Variably-Twinned Structure of a Novel Calmodulin-Peptide Complex in a Novel Configuration

Released:
DOI: 10.2210/pdb5dow/pdb
PDB entry 5dow coloured by chain, front view.
PDB entry 5dow coloured by chain, side view.
PDB entry 5dow coloured by chain, top view.
Source organisms:
Primary publication:
Solution of the structure of a calmodulin-peptide complex in a novel configuration from a variably twinned data set.
Keller JP
Acta Crystallogr D Struct Biol 73 22-31 (2017)
PMID: 28045382

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143967 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 148 amino acids
Theoretical weight: 16.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP23 (Residues: 2-149; Coverage: 99%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Chloride anion exchanger Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 22 amino acids
Theoretical weight: 2.56 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q9WVC8 (Residues: 563-583; Coverage: 3%)
Gene names: Dra, Slc26a3

Ligands and Environments

4 bound ligands:
Ligand SO4 2 x SO4
Ligand CA 16 x CA
Ligand CL 11 x CL
Ligand NA 5 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P3212
Unit cell:
a: 98.874Å b: 98.874Å c: 128.583Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.099 0.097 0.141
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 citations in other articles

Perfect merohedral twinning combined with noncrystallographic symmetry potentially causes the failure of molecular replacement with low-homology search models for the flavin-dependent halogenase HalX from Xanthomonas campestris.
Buss et al. (2018)

2 mentions without citation

Effect of Ca2+ on the promiscuous target-protein binding of calmodulin.
Westerlund et al. (2018)
1 more