› 5dy1

X-ray diffraction
2.65Å resolution

Crystal of Selenomethionine substituted AmtR from Corynebacterium glutamicum

Released:
DOI: 10.2210/pdb5dy1/pdb
PDB entry 5dy1 coloured by chain, front view.
PDB entry 5dy1 coloured by chain, side view.
PDB entry 5dy1 coloured by chain, top view.
Source organism: Corynebacterium glutamicum ATCC 14067
Primary publication:
Structure of AmtR, the global nitrogen regulator of Corynebacterium glutamicum, in free and DNA-bound forms.
Palanca C, Rubio V
FEBS J 283 1039-59 (2016)
PMID: 26744254

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TetR family transcriptional regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 24.64 KDa
Source organism: Corynebacterium glutamicum ATCC 14067
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R3
Unit cell:
a: 160.464Å b: 160.464Å c: 52.435Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.18 0.221
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
7 other articles

2 mentions without citation

Structure of AmtR, the global nitrogen regulator of Corynebacterium glutamicum, in free and DNA-bound forms.
Palanca et al. (2016)
1 more