› 5dza

X-ray diffraction
2.19Å resolution

Streptococcus agalactiae AgI/II polypeptide BspA C terminal domain (WT)

Released:
DOI: 10.2210/pdb5dza/pdb
PDB entry 5dza coloured by chain, front view.
PDB entry 5dza coloured by chain, side view.
PDB entry 5dza coloured by chain, top view.
Source organism: Streptococcus agalactiae NEM316
Primary publication:
Structural and Functional Analysis of Cell Wall-anchored Polypeptide Adhesin BspA in Streptococcus agalactiae.
Rego S, Heal TJ, Pidwill GR, Till M, Robson A, Lamont RJ, Sessions RB, Jenkinson HF, Race PR, Nobbs AH
J Biol Chem 291 15985-6000 (2016)
PMID: 27311712

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gram-positive cocci surface proteins LPxTG domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 36.49 KDa
Source organism: Streptococcus agalactiae NEM316
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8E589 (Residues: 554-881; Coverage: 37%)
Gene name: gbs1143
Sequence domains:
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2221
Unit cell:
a: 34.03Å b: 205.8Å c: 120.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.218 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Group B Streptococcal Maternal Colonization and Neonatal Disease: Molecular Mechanisms and Preventative Approaches.
Patras et al. (2018)
6 more

3 mentions without citation

The Multifaceted Nature of Streptococcal Antigen I/II Proteins in Colonization and Disease Pathogenesis.
Manzer et al. (2020)
2 more