› 5ecn

X-ray diffraction
1.72Å resolution

Crystal Structure of FIN219-FIP1 complex with JA, Leu and ATP

Released:
DOI: 10.2210/pdb5ecn/pdb
PDB entry 5ecn coloured by chain, front view.
PDB entry 5ecn coloured by chain, side view.
PDB entry 5ecn coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation.
Chen CY, Ho SS, Kuo TY, Hsieh HL, Cheng YS
Proc Natl Acad Sci U S A 114 E1815-E1824 (2017)
PMID: 28223489

Function and Biology Details

Reactions catalysed: 
ATP + jasmonate + an L-amino acid = AMP + diphosphate + a jasmonoyl-L-amino acid
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185262 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Jasmonoyl--L-amino acid synthetase JAR1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 575 amino acids
Theoretical weight: 64.42 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SKE2 (Residues: 1-575; Coverage: 100%)
Gene names: At2g46370, F11C10.6, FIN219, JAR1
Sequence domains: GH3 auxin-responsive promoter
Glutathione S-transferase U20 Chains: B, C, E, F
Molecule details ›
Chains: B, C, E, F
Length: 223 amino acids
Theoretical weight: 25.87 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8L7C9 (Residues: 1-217; Coverage: 100%)
Gene names: At1g78370, F3F9.11, FIP1, GSTU20
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 4 x GSH
3 bound ligands:
Ligand JAA 2 x JAA
Ligand LEU 2 x LEU
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P1
Unit cell:
a: 53.833Å b: 53.877Å c: 196.79Å
α: 92.27° β: 97.16° γ: 113.6°
R-values:
R R work R free
0.171 0.169 0.188
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

3 review citations

Jasmonates: News on Occurrence, Biosynthesis, Metabolism and Action of an Ancient Group of Signaling Compounds.
Wasternack et al. (2018)
2 more

2 mentions without citation

Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases.
Sylvestre-Gonon et al. (2019)
1 more