› 5eey

X-ray diffraction
1.98Å resolution

RADIATION DAMAGE TO THE TRAP-RNA COMPLEX: DOSE (DWD) 11.6 MGy

Released:
DOI: 10.2210/pdb5eey/pdb
PDB entry 5eey coloured by chain, front view.
PDB entry 5eey coloured by chain, side view.
PDB entry 5eey coloured by chain, top view.
Source organisms:
Primary publication:
RNA protects a nucleoprotein complex against radiation damage.
Bury CS, McGeehan JE, Antson AA, Carmichael I, Gerstel M, Shevtsov MB, Garman EF
OpenAccess logo Acta Crystallogr D Struct Biol 72 648-57 (2016)
PMID: 27139628

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo undecamer
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-578555 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Transcription attenuation protein MtrB Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V
Length: 74 amino acids
Theoretical weight: 8.26 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X6J6 (Residues: 1-74; Coverage: 100%)
Gene name: mtrB
Sequence domains: Tryptophan RNA-binding attenuator protein
Structure domains: TRAP-like
(GAGUU)10GAG 53-NUCLEOTIDE RNA Chain: W
Molecule details ›
Chain: W
Length: 55 nucleotides
Theoretical weight: 17.91 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand TRP 22 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 141.12Å b: 111.08Å c: 138.09Å
α: 90° β: 117.4° γ: 90°
R-values:
R R work R free
0.218 0.216 0.248
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 citation in other articles

Structure of the bacterial ribosome at 2 Å resolution.
Watson et al. (2020)
3 more

2 mentions without citation

OH cleavage from tyrosine: debunking a myth.
Bury et al. (2017)
1 more