› 5ef4

X-ray diffraction
2.05Å resolution

2.05 A crystal structure of the Amb a 11 cysteine protease, a major ragweed pollen allergen, in its proform

Released:
DOI: 10.2210/pdb5ef4/pdb
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PDB entry 5ef4 coloured by chain, front view.
PDB entry 5ef4 coloured by chain, side view.
PDB entry 5ef4 coloured by chain, top view.
Source organism: Ambrosia artemisiifolia
Primary publication:
Structural and Functional Characterization of the Major Allergen Amb a 11 from Short Ragweed Pollen.
Groeme R, Airouche S, Kopečný D, Jaekel J, Savko M, Berjont N, Bussieres L, Le Mignon M, Jagic F, Zieglmayer P, Baron-Bodo V, Bordas-Le Floch V, Mascarell L, Briozzo P, Moingeon P
J Biol Chem 291 13076-87 (2016)
PMID: 27129273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197617 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine protease Amb a 11.0101 Chains: A, B
Molecule details ›
Chains: A, B
Length: 385 amino acids
Theoretical weight: 43.08 KDa
Source organism: Ambrosia artemisiifolia
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: V5LU01 (Residues: 23-386; Coverage: 100%)
Sequence domains:
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P3121
Unit cell:
a: 117.68Å b: 117.68Å c: 101.73Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.17 0.199
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Ragweed Pollen Allergy: Burden, Characteristics, and Management of an Imported Allergen Source in Europe.
Chen et al. (2018)
3 more

3 mentions without citation

Plant and Arthropod IgE-Binding Papain-like Cysteine Proteases: Multiple Contributions to Allergenicity.
Giangrieco et al. (2024)
2 more

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