› 5etu

X-ray diffraction
2.53Å resolution

Cetuximab Fab in complex with L5E meditope variant

Released:
DOI: 10.2210/pdb5etu/pdb
PDB entry 5etu coloured by chain, front view.
PDB entry 5etu coloured by chain, side view.
PDB entry 5etu coloured by chain, top view.
Source organisms:
Primary publication:
Natural and non-natural amino-acid side-chain substitutions: affinity and diffraction studies of meditope-Fab complexes.
Bzymek KP, Avery KA, Ma Y, Horne DA, Williams JC
OpenAccess logo Acta Crystallogr F Struct Biol Commun 72 820-830 (2016)
PMID: 27834791

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-229998 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cetuximab Fab light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 213 amino acids
Theoretical weight: 23.29 KDa
Source organism: Mus musculus
Expression system: unidentified
Structure domains: Immunoglobulins
Cetuximab Fab heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 220 amino acids
Theoretical weight: 23.64 KDa
Source organism: Mus musculus
Expression system: unidentified
Structure domains: Immunoglobulins
L5E meditope variant Chains: E, F
Molecule details ›
Chains: E, F
Length: 12 amino acids
Theoretical weight: 1.49 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PO4 5 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 64.38Å b: 82.87Å c: 213Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.232
Expression systems:
  • unidentified
  • Not provided

Quick links

Citations

4 citation in other articles

A Platform To Enhance Quantitative Single Molecule Localization Microscopy.
Golfetto et al. (2018)
3 more

1 mention without citation

Natural and non-natural amino-acid side-chain substitutions: affinity and diffraction studies of meditope-Fab complexes.
Bzymek et al. (2016)