› 5f29

X-ray diffraction
1.82Å resolution

Structure of RCK domain with cda

Released:
DOI: 10.2210/pdb5f29/pdb
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PDB entry 5f29 coloured by chain, front view.
PDB entry 5f29 coloured by chain, side view.
PDB entry 5f29 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural Insights into the Distinct Binding Mode of Cyclic Di-AMP with SaCpaA_RCK.
Chin KH, Liang JM, Yang JG, Shih MS, Tu ZL, Wang YC, Sun XH, Hu NJ, Liang ZX, Dow JM, Ryan RP, Chou SH
Biochemistry 54 4936-51 (2015)
PMID: 26171638

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173488 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sodium:proton antiporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 73 amino acids
Theoretical weight: 8.79 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli O103:H2 str. 12009
UniProt:
  • Canonical: Q2FZQ4 (Residues: 542-614; Coverage: 12%)
Gene name: SAOUHSC_00946
Sequence domains: TrkA-C domain
Structure domains: Regulator of K+ conductance, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand 2BA 1 x 2BA
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P212121
Unit cell:
a: 41.489Å b: 47.297Å c: 70.174Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.201 0.255
Expression system: Escherichia coli O103:H2 str. 12009

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Citations

12 review citations

Cyclic di-GMP: second messenger extraordinaire.
Jenal et al. (2017)
11 more

5 mentions without citation

Cyclic di-AMP, a second messenger of primary importance: tertiary structures and binding mechanisms.
He et al. (2020)
4 more

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