› 5ffr

X-ray diffraction
2.2Å resolution

Crystal Structure of Surfactant Protein-A complexed with phosphocholine

Released:
DOI: 10.2210/pdb5ffr/pdb
PDB entry 5ffr coloured by chain, front view.
PDB entry 5ffr coloured by chain, side view.
PDB entry 5ffr coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Elucidation of Lipid Binding Sites on Lung Surfactant Protein A Using X-ray Crystallography, Mutagenesis, and Molecular Dynamics Simulations.
Goh BC, Wu H, Rynkiewicz MJ, Schulten K, Seaton BA, McCormack FX
Biochemistry 55 3692-701 (2016)
PMID: 27324153

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pulmonary surfactant-associated protein A Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.7 KDa
Source organism: Rattus norvegicus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P08427 (Residues: 101-248; Coverage: 65%)
Gene names: Sftp-1, Sftp1, Sftpa, Sftpa1
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand PC 1 x PC
Ligand NA 1 x NA
1 modified residue:
Ligand SME 2 x SME

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P63
Unit cell:
a: 97.349Å b: 97.349Å c: 44.645Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.169 0.193
Expression system: Trichoplusia ni

Quick links

Citations

3 review citations

Characterization of Lipid-Protein Interactions and Lipid-Mediated Modulation of Membrane Protein Function through Molecular Simulation.
Muller et al. (2019)
2 more

3 mentions without citation

Elucidation of Lipid Binding Sites on Lung Surfactant Protein A Using X-ray Crystallography, Mutagenesis, and Molecular Dynamics Simulations.
Goh et al. (2016)
2 more